ŋ߂̔­•\˜_•¶ (1995`)


"Loading direction regulates the affinity of ADP for kinesin"
Uemura, S. and Ishiwata, S. (2003).
Nature Struct Biol. 4, 308-311.

PDF(517KB)

"Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics"
Kawaguchi,K., Uemura, S., and Ishiwata, S. (2003).
Biophys. J. 84, 1103-1113.

PDF(323KB)

"Direct observation of polymerization-depolymerization dynamics of single actin filaments."
Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T. and Ishiwata, S. (2002).
Nature Cell Biol. 4, 666-673.

PDF(366KB) Movie (gif)

"Regulatory roles of light chain 2 in MgADP-induced contraction."
Fujita, H., Sasaki, D., Fukuda, K. and Ishiwata, S. (2002).
J. Physiol. 542, 221-229.

"Myosin V is a left-handed spiral motor on the right-handed actin helix."
M. Yusuf Ali, Uemura, S., Adachi, K., Itoh, H., Kinosita, Jr., K. and Ishiwata, S. (2002).
Nature Struct. Biol. 9, 464-467.

PDF(1,759KB)

"Visualization and force measurement of branching by Arp2/3 complex and N-WASP in actin filament."
Fujiwara, I., Suetsugu, S., Uemura, S., Takenawa, T. and Ishiwata, S. (2002). Biochem. Biophys. Res. Comm. 293, 1550-1555.

PDF(370KB)Movies

"Kinesin-microtubule binding is dependent on both nucleotide state and loading direction."
Uemura, S., Kawaguchi, K., Yajima, J., Edamatsu, M., Toyoshima, Y. Y. and Ishiwata, S. (2002).
Proc. Natl. Acad. Sci. USA. 99, 5977-5981.

PDF(417KB)

"Elementary steps of the cross-bridge cycle in bovine myocardium with and without regulatory proteins."
Fujita, H., Sasaki, D., Ishiwata, S. and Kawai, M. (2002).
Biophys. J. 82, 915-928.

Length dependence of tension generation in rat skinned cardiac muscle: Role of titin in the Frank-Starling mechanism of the heart.
Fukuda, N., Sasaki, D., Ishiwata, S. and Kurihara, S.Circulation 104:1639-1645 (2001).

Acidosis and inorganic phosphate enhance length dependence of tension generation in skinned rat cardiac muscle.
Fukuda, N., Ouchi, J., Kajiwara, H., Sasaki, D., Ishiwata, S. and Kurihara, S.J. Physiol. 536:153-160 (2001).

Thermal activation of single kinesin molecules with temperature pulse microscopy.
Kawaguchi, K. and Ishiwata, S.Cell Motil. Cytoskel. 49: 41-47 (2001).

"DNAƒvƒ[ƒuƒAƒŒƒC‚ð—p‚¢‚½DNA’f•ЉñŽû"(Recovery of DNA Fragment from a DNA Probe Array)
‰ª–ì˜aéA’Â|A¬Œ´Œ«MAŠŽR’q°AˆÀ“cŒ«“ñAΓnMˆê “d‹CŠw‰ïŽiƒZƒ“ƒTEƒ}ƒCƒNƒƒ}ƒVƒ“•”–åŽjTrans. IEE of Japan 121-E, No.4: 181-186 (2001).

Nucleotide-dependent single- to double-headed binding of kinesin.
Kawaguchi, K. and Ishiwata, S. Science 291: 667-669 (2001).

Microscopic analysis of polymerization and fragmentation of individual actin filaments.
Ishiwata,S., Tadashige,J., Masui,I., Nishizaka,T. and Kinosita,Jr.,K. In Molecular Interactions of Actin: Actin Structure and Actin-binding Proteins. (eds. C.G. dos Remedios and D.D. Thomas) Springer Verlag, Heidelberg : 79-94 (2001).


Position-specific release of DNA from a chip by using photothermal denaturation

K. Okano, K. Yasuda and S. Ishiwata Sensors & Actuators B 64: 88-94. (2000).

Characterization of single actomyosin rigor bonds - Load-dependence of lifetime and mechanical properties-

T. Nishizaka, R. Seo, H. Tadakuma, K. Kinosita Jr. and S. Ishiwata Biophys. J. 79(2): 962-974 (2000).

Temperature dependence of force, velocity and processivity of single kinesin molecules

K. Kawaguchi and S. Ishiwata Biochem. Biophys. Res. Comm. 272: 895-899 (2000).

Temperature change does not affect force between single actin filaments and HMM from rabbit muscles.

M. Kawai, K. Kawaguchi, M. Saito and S. Ishiwata Biophys. J. 78: 3112-3119 (2000).

¶‘Ì•ªŽqƒ‚[ƒ^[Œn‚É‚¨‚¯‚镪ŽqƒVƒ“ƒNƒƒiƒCƒ[[ƒVƒ‡ƒ“‚ÌŒ¤‹† (Investigation of molecular synchronization in the assembly of molecular motors)


“¡“c‰p–¾A²X–Ø‘å•ãAΓnMˆê@ƒoƒCƒIƒTƒCƒGƒ“ƒX‚ƃCƒ“ƒ_ƒXƒgƒŠ[ 58: 256-259 (2000).

‹ØŽûkŠŠ‚è‰^“®‹@\‚ƃgƒ‰ƒCƒ{ƒƒW[ (Sliding mechanism of muscle contraction and tribology)

ΓnMˆê ƒgƒ‰ƒCƒ{ƒƒWƒXƒg 45: 119-125. (2000).

Microscopic analysis of polymerization and fragmentation of individual actin filaments

S. Ishiwata, J. Tadashige, I. Masui, T. Nishizaka and K. Kinosita Jr. In Molecular Interactions of Actin: Actin Structure and Actin-binding Proteins. (eds. C.G. dos Remedios and D.D. Thomas) Springer Verlag, Heidelberg, in press. (2000).

Focal extraction of surface-bound DNA from a microchip using photo-thermal denaturation

K. Yasuda, K. Okano. and S. Ishiwata Biotechniques 28: 1006-1011 (2000).

Effects of MgADP on length dependence of tension generation in skinned rat cardiac muscle

N. Fukuda, H. Kajiwara, S. Ishiwata and S. Kurihara Circ.Res. 86: e1-e6 (2000).

Tropomyosin modulates pH dependence of isometric tension

H. Fujita and S. Ishiwata Biophys.J. 77: 1540-1546 (1999).

Imaging of thermal activation of actomyosin motors

H. Kato, T. Nishizaka, T. Iga, K. Kinosita Jr. and S. Ishiwata Proc.Natl.Acad. Sci.USA 96: 9602-9606 (1999).

Effects of pH on Spontaneous Tension Oscillation in Skinned Bovine Cardiac Muscle

N. Fukuda, and S. Ishiwata Pflugers Arch. (Eur. J. Physiol.) 438: 125-132 (1999).

Contractile Propertiles of Thin (Actin) Filament-Reconstituted Muscle Fibers

in Mechanisms of Work Production and Work Absorption in Muscle (eds. H. Sugi & G. Pollack) S. Ishiwata, T. Funatsu, and H. Fujita Plenum Press (1998).

Regulatory Roles of MgADP and Calcium in Tension Development of Skinned Cardiac Muscle

H. Fukuda, H. Fujita, T. Fujita, and S. Ishiwata J. Muscle Res. Cell Motil. 19: 909-921 (1998).

Spontaneous Oscillatory Contraction Without Regulatory Proteins in Actin Filament-Reconstituted Fibers

H. Fujita, and S. Ishiwata Biophys. J. 75: 1439-1445 (1998).

F1-ATPase: A Rotary Motor Made of a Single Molecule

K. Kinosita Jr., R. Yasuda, H. Noji, S. Ishiwata, and M. Yoshida Cell 93: 21-24 (1998).

The Use of Fluorescent Probes

S. Ishiwata Current Methods in Muscle Physiology (ed. H. Sugi) Oxford University Press: 199-221 (1998).

S‹Ø‚ÌŽûk‚Æ’´•ªŽq‚ÌŽ©ŒÈ‘gD‰»

ΓnMˆê ‰ÈŠw 68: 110-113 (1998).

Interhead Distances in Myosin Attached to F-Actin Estimated by Fluorescence Energy Transfer Spectroscopy

S. Ishiwata, M. Miki, S. Izumi, T. Funatsu, K. Yasuda, and C. dos Remedios Biophys. J. 73: 895-904 (1997).

Axial Rotation of Sliding Actin Filaments Revealed by Single-fluorophore Imaging

I. Sase, H. Miyata, S. Ishiwata, and K. Kinosita Jr. Proc. Natl. Acad. Sci. USA 94: 5646-5650 (1997).

¶‘ÌŒnÞ—¿‚Ì•ªŽqƒVƒ“ƒNƒƒiƒCƒ[[ƒVƒ‡ƒ“

ΓnMˆê ƒ|ƒŠƒtƒ@ƒCƒ‹ 34: 25-29 (1997).

Two-Dimensional Arrangement of a Functional Protein by Cysteine-Gold Interaction: Enzyme Activity and Characterization of a Protein Monolayer on a Gold Substrate

Y.C. Sasaki, K. Yasuda, Y. Suzuki, T. Ishibashi, I. Satoh, Y. Fujiki, and S. Ishiwata Biophys. J. 72: 1842-1848 (1997).

Microscopic Analysis of the Nature of Forces in a Single Actomyosin Motor and its Assemblage

S. Ishiwata, T. Nishizaka, H. Kato, H. Tadakuma, T. Iga, N. Suzuki, H. Miyata, and K. Kinosita Jr. Neurochemistry (Moscow) 13: 305-312 (1996).

Spontaneous Tension Oscillation in Skinned Bovine Cardiac Muscle

N. Fukuda, H. Fujita, T. Fujita, and S. Ishiwata Pflugers Arch. (Eur. J. Physiol.) 433: 1-8 (1996).

Structural and Functional Reconstitution of Thin Filaments in the Contractile Apparatus of Cardiac Muscle

H. Fujita, K. Yasuda, S. Niitsu, T. Funatsu, and S. Ishiwata Biophys. J. 71: 2307-2318 (1996).

Ca2+-Induced Tension Development in the Stalks of Glycerinated Vorticella Convallaria

Y. Moriyama, K. Yasuda, S. Ishiwata, and H. Asai Cell Motil. & Cytoskel. 34: 271-278 (1996).

Microscopic Analysis of the Elastic Properties of Connectin/titin and Nebulin in Myofibrils

S. Ishiwata, K. Yasuda, Y. Shindo, and H. Fujita Adv. Biophys. 33: 135-142 (1996).

Soft-X-ray Damage to Biological Samples

H. Fujisaki, S. Takahashi, H. Ohzeki, K. Sugisaki, H. Kondo, H. Nagata, H. Kato, and S. Ishiwata J. Microscopy 182: 79-83 (1996).

Synchronous Behavior of Spontaneous Oscillations of Sarcomeres in Skeletal Myofibrils under Isotonic Conditions

K. Yasuda, Y. Shindo, and S. Ishiwata Biophys. J. 70: 1823-1829 (1996).

•ªŽqƒ‚[ƒ^[‚Ì—ÍŠwE‹@”\“Á«‚ðŒ©‚é

¼â’”V, ΓnMˆê ¶•¨•¨— 203: 15-19 (1996).

Unbinding Force of a Single Motor Molecule of Muscle Measured Using Optical Tweezers

T. Nishizaka, H. Miyata, H. Yoshikawa, S. Ishiwata, and K. Kinosita Jr. Nature 377: 251-254 (1995).

‹ØŽûkŒn‚Ì—ÍŠw“Á«

ΓnMˆê BME (“ú–{MEŠw‰ïj 9: 12-20 (1995).

Mechanical Properties of Single Protein Motor of Muscle Studied by Optical Tweezers

T. Nishizaka, H. Miyata, H. Yoshikawa, S. Ishiwata, and K. Kinosita Jr. Biophys. J. 68: 75s (1995).

Mechanical Measurements of Single Actomyosin Motor Force

H. Miyata, H. Yoshikawa, H. Hakozaki, N. Suzuki, T. Furuno, A. Ikegami, K. Kinosita Jr., T. Nishizaka, and S. Ishiwata Biophys. J. 68: 286s-290s (1995).

Microscopic Analysis of the Elastic Properties of Nebulin in Skeletal Myofibrils

K. Yasuda, T. Anazawa, and S. Ishiwata Biophys. J. 68: 598-608 (1995).