Japanese / English
Current Published Papers
2012
"Protein-protein interactions in solution and their interplay with protein specific functions"
Takaaki Sato, Toshiko Fukasawa, Togo Shimozawa, Teruyuki Komatsu, Hiromi Sakai, and Shin'ichi Ishiwata (2012)
J. Phys. Soc. Jpn. 81, SA002
"Improvement of the yields of recombinant actin and myosin V-HMM in the insect cell/baculovirus system by the addition of nutrients to the high-density cell culture"
Takashi Ohki, Sergey V. Mikhailenko, Tomomi Arai, Shuya Ishii, and Shin'ichi Ishiwata (2012)
J. Muscle Res. Cell Motil. 33(5), 351-358
"Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled library"
Satoshi Arai, Shota Hirosawa, Yusuke Oguchi, Madoka Suzuki, Atsushi Murata, Shin'ichi Ishiwata, and Shinji Takeoka (2012)
ACS Combinatorial Science On-line, doi:10.1021/co300028n
"Mechanical impulses can control metaphase progression in a mammalian cell."
Takeshi Itabashi, Yasuhiko Terada, Kenta Kuwana, Tetsuo Kan, Iswao Shimoyama, and Shin'ichi Ishiwata (2012)
Proc. Natl. Acad. Sci. U.S.A. 109(19), 7320-7325
"Walking nanothermometers: Spatiotemporal temperature measurement of transported acidic organelles in single living cells."
Kotaro Oyama, Masao Takabayashi, Yoshiaki Takei, Satoshi Arai, Shinji Takeoka, Shin'ichi Ishiwata, and Madoka Suzuki (2012)
Lab Chip 12(9), 1591-1593, doi:10.1039/C2LC00014H
"Sarcomere imaging by quantum dots for the study of cardiac muscle physiology"
Fuyu Kobirumaki-Shimozawa, Kotaro Oyama, Takahiro Serizawa, Akari Mizuno, Tatsuya Kagemoto, Togo Shimozawa, Shin'ichi Ishiwata, Satoshi Kurihara, and Norio Fukuda (2012)
J. Biomed. Biotech. 2012, 313814, doi:10.1155/2012/313814
"Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients"
Kotaro Oyama, Akari Mizuno, Seine A. Shintani, Hideki Itoh, Takahiro Serizawa, Norio Fukuda, Madoka Suzuki, and Shin'ichi Ishiwata. (2012)
Biochem. Biophys. Res. Commun. 417, 607-612
2011
"Quasi-periodic distribution of rigor cross-bridges along areconstituted thin filament in a skeletal myofibril"
Madoka Suzuki and Shin'ichi Ishiwata (2011).
Biophys. J. 101, 2740-2748.
"The role of tropomyosin domains in cooperative activation of theactin-myosin interaction"
Yusuke Oguchi, Junji Ishizuka, Sarah E Hitchcock-DeGregori, Shin'ichi Ishiwata, and Masataka Kawai (2011).
J. Mol. Biol. 414, 667-680.
"Sarcomere length-dependent Ca2+ activation in skinned rabbit psoas muscle fibers: coordinated regulation of thin filament cooperative activation and passive force."
Norio Fukuda, Takahiro Inoue, Mitsunori Yamane, Takako Terui, Fuyu Kobirumaki, Iwao Ohtsuki, Shin'ichi Ishiwata, and Satoshi Kurihara (2011)
J. Physiol. Sci. 61 (6), 515-523
"Real-time measurement of the length of a single sarcomere in rat ventricular myocytes: a novel analysis with quantum dots"
Takahiro Serizawa, Takako Terui, Tatsuya Kagemoto, Akari Mizuno, Togo Shimozawa, Fuyu Kobirumaki, Shin'ichi Ishiwata, Satoshi Kurihara, and Norio Fukuda (2011).
Am. J. Physiol. Cell Physiol. doi:10.1152/ajpcell.00161.2011
"Depressed contractile performance and reduced fatigue resistance insingle skinned fibers of soleus muscle after long-term disuse in rats"
Jun Udaka, Takako Terui, Iwao Ohtsuki, Keishi Marumo, Shin'ichi Ishiwata, Satoshi Kurihara, and Norio Fukuda (2011).
J. Appl. Physiol. doi:10.1152/japplphysiol.00330.2011
"Insights into the micro-mechanical properties of the metaphase spindle"
Yuta Shimamoto, Yusuke T. Maeda, Shin'ichi Ishiwata, Albert J. Libchaber, and Tarun M. Kapoor (2011).
Cell 145, 1062-1074.
"The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends."
Yusuke Oguchi, Seiichi Uchimura, Takashi Ohki, Sergey V. Mikhailenko, and Shin'ichi Ishiwata (2011)
Nature Cell Biol. 13, 846-852
"SPontaneous Oscillatory Contraction (SPOC): auto-oscillations observed in striated muscle at partial activation"
James Erle Wolfe, Shin'ichi Ishiwata, Filip Braet, Renee Whan, Yingying Su, Sean Lal, and Cristobal G. dos Remedios. (2011)
Biophys. Rev. 3, 53-62
"A theory on auto-oscillation and contraction in striated muscle."
Katsuhiko Sato, Masako Ohtaki, Yuta Shimamoto, and Shin'ichi Ishiwata. (2011).
Prog. Biophys. Mol. Biol. 105, 199-207
"Contractile system of muscle as an auto-oscillator."
Shin'ichi Ishiwata, Yuta Shimamoto,* and Norio Fukuda.* (2011). * equal contribution
Prog. Biophys. Mol. Biol. 105, 187-198.
"Two-dimensional periodic texture of actin filaments formed upon drying."
Hajime Honda and Shin'ichi Ishiwata (2011). BIOPHYSICS 7, 11-19.PDF(2070KB)
2010
"Regulatory mechanism of length-dependent activation in skinned porcineventricular muscle: role of thin filament cooperative activation in theFrank-Starling relation."
Takako Terui*, Yuta Shimamoto*, Mitsunori Yamane, Fuyu Kobirumaki, IwaoOhtsuki, Shin'ichi Ishiwata, Satoshi Kurihara, and Norio Fukuda. (2010). * Equal contribution.
J. Gen. Physiol. 136, 469-482
"Modulation of the mechano-chemical properties of myosin V by drebrin-E."
Hiroaki Kubota, Ryoki Ishikawa, Takashi Ohki, Junji Ishizuka, Sergey V. Mikhailenko and Shin'ichi Ishiwata. (2010).
Biochem. Biophys. Res. Commun. 400(4), 643-648
"Molecular motors as an auto-oscillator."
Shin'ichi Ishiwata, Yuta Shimamoto and Madoka Suzuki. (2010).
HFSP J. 4(3), 100-104.PDF(215KB)
"A Potential Role of Actin Filament Polymerization/Depolymerization in Smooth Muscle Contraction."
Masaru Watanabe, Shin'ichi Ishiwata, and Ying-Ming Liou. (2010).
Adapt. Med. 2(1), 23-28.
"Insights into the mechanisms of myosin and kinesin molecular motors from the single-molecule unbinding force measurements."
Sergey V. Mikhailenko, Yusuke Oguchi, and Shin'ichiIshiwata. (2010).
J Royal Soc. Interface. 7, S295-S306
"Robust processivity of myosin V under off-axis loads."
Yusuke Oguchi, Sergey V Mikhailenko, Takashi Ohki, Adrian O Olivares, Enrique M De La Cruz& Shin'ichi Ishiwata. (2010).
Nature Chemical Biology.6(4): 300-305; doi:10.1038/nchembio.322
"Key residues on microtubule responsible for activation of kinesin ATPase."
Uchimura S*, Oguchi Y*, Hachikubo Y, Ishiwata S, Muto E. (2010).
* Equal contribution.
EMBO J. 29, 1167-1175; doi:10.1038/emboj.2010.25
"Titin-based regulations of diastolic and systolic functions ofmammalian cardiac muscle."
Norio Fukuda, Takako Terui, Shin'ichi Ishiwata and Satoshi Kurihara. (2010).
J. Mol. Cell. Cardiol. 48(5) :876-881
"Activated protein C prevents glutamate- and thrombin-induced activationof nuclear factor-?EB in cultured hippocampal neurons."
L. Gorbacheva, V.Pinelis, S. Ishiwata, S. Strukova and G. Reiser. (2010).
Neurosci. 165(4), 1138-1146.
2009
"Actin oligomers at the initial stage of polymerization induced by increasing temperature under low ionic strength: Study with Small-angle X-ray scattering."
Takaaki Sato*, Togo Shimozawa*, Toshiko Fukasawa, Masako Ohtaki, Kenji Aramaki, Katsuzo Wakabayashi and Shin'ichi Ishiwata. (2009).
* Equal contribution.
BIOPHYSICS. 6, 1-11.
"D-loop of actin differently regulates the motor function of myosins II and V."
Hiroaki Kubota, Sergey V. Mikhailenko, Harumi Okabe, Hideki Taguchi, and Shin'ichi Ishiwata. (2009).
J. Biol. Chem. 284, 35251-35258
"Endothelial protein C receptor is expressed in rat cortical and hippocampal neurons and isnecessary for protective effect of activated protein C at glutamate excitotoxicity."
Lyubov Gorbacheva, Olga Davidova, Elena Sokolova, Shin'ichi Ishiwata, Vsevolod Pinelis,Svetlana Strukova and Georg Reiser. (2009).
J. Neurochem. Nov;111(4), 967-75. Epub 2009 Sep 25.
"Inter-sarcomere coordination in muscle revealed through individual sarcomere response to quick stretch."
Yuta Shimamoto, Madoka Suzuki, Sergey V. Mikhailenko, Kenji Yasuda, and Shin'ichi Ishiwata. (2009).
Proc. Natl. Acad. Sci. U.S.A. 106, 11954-11959
"Protein kinase A-dependent modulation of Ca2+ sensitivity in fast skeletal muscle reconstituted with cardiac troponin."
Matsuba, D., Terui, T., O-Uchi, J., Tanaka, H., Ojima, T., Ohtsuki, I., Ishiwata, S., Kurihara, S. and Fukuda, N. (2009).
J. Gen. Physiol. 133(6), 571-581.
"Purification ofcytoplasmic actin by affinity chromatography using the C-terminal half of gelsolin."
Ohki,T., Ohno, C., Oyama, K., Mikhailenko, S.V. and Ishiwata, S. (2009).
Biochem. Biophys. Res. Commun. 383(1), 146-50.
"Regulatory mechanism of smooth muscle contraction studied with gelsolin-treated strips ofTaenia Caeci in Guinea Pig."
Liou, Y.-M., Watanabe, M., Yumoto, M. and Ishiwata, S. (2009).
Am. J. Physiol. Cell Physiol. 296: C1024-C1033.
"Probing the mechanical architecture of the vertebrate meiotic spindle."
Itabashi, T., Takagi, J., Shimamoto, Y., Onoe, H., Kuwana, K., Shimoyama, I., Gaetz, J., Kapoor, T. M. and Ishiwata, S. (2009).
Nature Methods. 6(2), 167-172.
"Highly thermosensitive Ca2+ dynamics in a HeLa cell through IP3 receptors."
Tseeb, V., Suzuki, M., Oyama, K., Iwai, K. and Ishiwata, S. (2009).
HFSP J. 3, 117-123.PDF(344KB)
"Mechanical distortion of single actinfilaments induced by external force: Detection by fluorescence imaging."
Shimozawa,T. and Ishiwata, S. (2009).
Biophys. J. 96, 1036-1044.
"Titin and troponin: central players in the Frank-Starling mechanism of the heart."
Fukuda, N. Terui, T. Ohtsuki, I. Ishiwata, S. and Kurihara, S. (2009).
Curr. Cardiol. Rev. 5, 119-124.
2008
"Load-dependent ADP binding to myosins V and VI: Implications for subunit coordination and function."
Yusuke Oguchi*, Sergey V. Mikhailenko*, Takashi Ohki, Adrian O. Olivares, Enrique M. De La Cruz, and Shin'ichi Ishiwata (2008).
* Equal contribution.
Proc. Natl. Acad. Sci. U.S.A. 105, 7714-7719.
"Mechanism of spontaneous oscillation emerging from collective molecular motors."
Shimamoto, Y. and Ishiwata, S (2008).
In Physics of Self-Organization Systems (ed. by S. Ishiwata and Y. Matsunaga), World Scientific, Singapore, 2008, pp. 47-56.
"Physiological functions of the giant elastic protein titin in mammalian striated muscle."
Fukuda, N., Granzier, H.L., Ishiwata, S. and Kurihara, S. (2008).
J. Physiol. Sci. 58, 151-159.PDF(4.27 MB)
"Activated protein C via PAR1 receptor regulates survival of neurons under conditions of glutamate excitotoxicity."
Gorbacheva, L.R., Storozhevykh, T.P., Pinelis, V.G., Davydova, O.N.,Ishiwata, S. and Strukova, S.M. (2008).
Biochemistry (Moscow) 73, 717-724.
"How load and the nucleotide state affect the actin filament binding mode of the molecular motor myosin V."
Mikhailenko, S. V., Oguchi, Y., Ohki T., Shimozawa, T., Olivares, A. O., De La Cruz, E. M. and Ishiwata, S. (2008).
J. Korean Phys. Soc. 53,1726-1730.PDF(2457KB)
"Troponin and titin coordinately regulate length-dependent activation in skinned porcine ventricular muscle."
Terui, T., Sodnomtseren, M., Matsuba, D., Udaka, J., Ishiwata, S., Ohtsuki, I., Kurihara, S. and Fukuda, N. (2008).
J. Gen. Physiol. 131, 275-283
"Disuse-induced Preferential Loss of the Giant Protein Titin Depresses Muscle Performance via Abnormal Sarcomeric Organization."
Udaka, J., Ohmori, S., Terui, T., Ohtsuki, I., Ishiwata, S., Kurihara, S. and Fukuda, N. (2008).
J. Gen. Physiol. 131, 33-41.Abstract
"Temperature Dependence of the Flexural Rigidity of Single Microtubules."
Kawaguchi, K., Ishiwata, S. and Yamashita, T. (2008).
Biochem. Biophys. Res. Commun. 366, 637-642.
"Length-dependent Activation and Auto-oscillation in Skeletal Myofibrils at PartialActivation by Ca2+."
Shimamoto, Y., Suzuki, M. and Ishiwata, S. (2008).
Biochem. Biophys. Res. Commun. 366, 233-238.
2007
"Protease-activated receptor (PAR)1-mediated anti-apoptotic effect of activated protein C on glutamateexcitotoxicity in hippocampal neurons."
L. R. Gorbacheva, T. P. Storozhevykh, V. G. Pinelis, S. Ishiwata, andS. M. Strukova (2007).
Thrombosis and Haemostasis, 98, 1150-1152.
"Non-linear force-length relationship in the ADP-induced contraction of skeletal myofibrils."
Yuta Shimamoto, Fumiaki Kono, Madoka Suzuki, and Shin'ichi Ishiwata (2007).
Biophys. J. 93, 4330-4341.Abstract
"Microscopic detection of thermogenesis in a single HeLa cell."
Madoka Suzuki, Vadim Tseeb, Kotaro Oyama, and Shin'ichi Ishiwata (2007).
Biophys. J. 92, L46-L48.Abstract
"Regulation of muscle contraction by Ca2+ and ADP: Focusing on the auto-oscillation (SPOC)."
Ishiwata, S., Shimamoto, Y., Suzuki, M. and Sasaki, D. (2007).
Adv. Exp. Med. Biol. 592, 341-358.
"Size distribution of linear and helical polymers in actin solution analyzed by photon counting histogram"
Terada, N., Shimozawa, T., Ishiwata, S. and Funatsu, T. (2007).
Biophys. J. 92, 2162-2171.Abstract
2006
"Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin."
Uchimura, S*., Oguchi, Y*., Katsuki, M*., Usui, T., Osada, H., Nikawa, J., Ishiwata, S. and Muto, E. (2006).
* Equal contribution.
EMBO J. 25, 5932-5941.Abstract
"Modulation of hippocampal neuron survival by thrombin and factor Xa."
Gorbacheva, L.R., Storozhevykh, T.P., Pinelis, V.G., Ishiwata, S. and Strukova, S.M.(2006).
Biochemistry (Moscow) 71, 1082-1089.Abstract
Main_PDF(212KB) Graph_PDF(3MB)
"Use of thin-filament reconstituted muscle fibres to probe the mechanism of force generation."
Kawai, M. and Ishiwata, S. (2006).
J. Muscle Res. Cell Motil. 27, 455-468. Abstract PDF(557KB)
"Temperature change does not affect force between regulated actin filaments and HMM in single molecule experiments."
Kawai, M., Kido, T., Vogel, M., Fink, R.H.A. and Ishiwata, S. (2006).
J.Physiol. 574.3, 877-887.Abstract
"Myocardial sarcomeres spontaneously oscillate with the period of heartbeat under physiological conditions."
Sasaki, D., Fukuda, N. and Ishiwata, S. (2006).
Biochem. Biophys. Res. Commun. 343, 1146-1152. Abstract
"Processivity of kinesin motility is enhanced on increasing temperature."
Nara, I. and Ishiwata, S. (2006).
BIOPHYSICS 2, 13-21. Abstract PDF(1327KB)
"Muscle proteins as high speed nano transporters on micro patterns."
Yoshida, Y., Shimozawa, T., Nishizaka, T., Ishiwata, S. and Takeuchi, S. (2006).
MEMS 2006 , 134-137.
2005
"Auto-oscillations of skinned myocardium correlating with heartbeat."
Sasaki, D., Fujita, H., Fukuda, N., Kurihara, S. and Ishiwata, S. (2005).
J. Muscle Res. Cell Motil. 26, 93-101.Abstract
"A new muscle contractile system composed of a thick filament lattice and a single actin filament."
Suzuki, M., Fujita, H. and Ishiwata, S. (2005).
Biophys. J. 89(1), 321-8. Abstract
"Molecular synchronization in actomyosin motors -From single molecules to muscle fibers via nanomuscle-."
Ishiwata, S., Shimamoto, Y., Sasaki, D. and Suzuki, M. (2005).
Adv. Exp. Med. Biol. 565, 25-36.
2004
"Mechanochemical coupling of two substeps in a single myosin V motor."
Uemura, S., Higuchi, H., Olivares, A.O., De La Cruz, E.M. and Ishiwata, S. (2004).
Nature Struct. Mol. Biol. 11, 877-883.Abstract PDF(995KB)
"A novel method of thermal activation and temperature measurement in the microscopic region around single living cells"
Zeeb, V., Suzuki, M. and Ishiwata, S. (2004).
J. Neurosci. Meth. 139(1), 69-77.Abstract
"A 1480/1064 nm dualwavelength photo-thermal etching system for non-contact three-dimensional microstructure generation into agarmicroculture chip"
Hattori, A., Moriguchi, H., Ishiwata, S. and Yasuda, K. (2004).
Sensors & Actuators B. 100, 467-474.
"Contractile systems of muscles"
Suzuki, M. and Ishiwata, S. (2004).
In Reflexive polymers and hydrogels: Understanding and designing fast-responsive polymeric systems. (ed. by N. Yui, R. Mrsny and K. Park), CRC Press LLC. pp. 33-48.
"The effect of tropomyosin on force and elementary steps of the cross-bridge cycle in reconstituted bovine myocardium"
Fujita, H., Lu, X., Suzuki, M., Ishiwata, S. and Kawai, M. (2004).
J. Physiol, 556.2, 637-649.Abstract
2003
"Molecular and cellular aspects of muscle contraction. General discussion part I"
Pollack, G. H., Ishiwata , S. and Sugi, H. (2003)
Adv. Exp. Med Biol., 538, 647-54.
"Bio-nanomuscle project: Contractile properties of single actin filaments in an A-bandmotility assay system"
Suzuki, M., Fujita, H. and Ishiwata, S. (2003).
In Molecular and cellular aspects of muscle contraction. (ed. by H. Sugi), Kluwer Acad./Plenum Press. pp.103-110.Abstract
"Loading direction regulates the affinity of ADP for kinesin"
Uemura, S. and Ishiwata, S. (2003).
Nature Struct Biol. 4, 308-311.Abstract
"Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics"
Kawaguchi,K., Uemura, S., and Ishiwata, S. (2003).
Biophys. J. 84, 1103-1113.
2002
"Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle."
Fujita, H., Sasaki, D., Fukuda, K. and Ishiwata, S. (2002).
J. Physiol. 542, 221-229.Abstract
"Myosin V is a left-handed spiral motor on the right-handed actin helix."
M. Yusuf Ali, Uemura, S., Adachi, K., Itoh, H., Kinosita, Jr., K. and Ishiwata, S. (2002).
Nature Struct. Biol. 9, 464-467.
"Kinesin-microtubule binding is dependent on both nucleotide state and loading direction."
Uemura, S., Kawaguchi, K., Yajima, J., Edamatsu, M., Toyoshima, Y. Y. and Ishiwata, S. (2002).
Proc. Natl. Acad. Sci. USA. 99, 5977-5981.
"Elementary steps of the cross-bridge cycle in bovine myocardium with and without regulatory proteins."
Fujita, H., Sasaki, D., Ishiwata, S. and Kawai, M. (2002).
Biophys. J. 82, 915-928.Abstract
2001
"Length dependence of tension generation in rat skinned cardiac muscle. Role of titin in the Frank-Starling mechaism of the heart."
Fukuda, N., Sasaki, D., Ishiwata, S. and Kurihara, S. (2001).
Circulation. 104, 1639-1645. Abstract
"Acidosis or inorganic phosphate enhances the length dependence of tension in rat skinned cardiac muscle."
Fukuda, N., Ouchi, J., Kajiwara, H., Sasaki, D., Ishiwata, S. and Kurihara, S. (2001).
J. Physiol. 536, 153-160.Abstract
"Thermal activation of single kinesin molecules with temperature pulse microscopy."
Kawaguchi, K. and Ishiwata, S. (2001).
Cell Motil. Cytoskel. 49, 41-47.Abstract
"Nucleotide-dependent single- to double-headed binding of kinesin."
Kawaguchi,K. and Ishiwata,S. (2001).
Science 291, 667-669. Abstract
"Microscopic analysis of polymerization and fragmentation of individual actin filaments."
Ishiwata,S., Tadashige,J., Masui,I., Nishizaka,T. and Kinosita,Jr.,K. (2001).
In Molecular Interactions of Actin: Actin Structure and Actin-binding Proteins. (eds. C.G. dos Remedios and D.D. Thomas) Springer Verlag, Heidelberg, pp. 79-94.
2000
"Characterization of single actomyosin rigor bonds - Load-dependence of lifetime and mechanical properties-."
Nishizaka,T., Seo,R., Tadakuma,H., Kinosita,Jr.,K. and Ishiwata,S. (2000).
Biophys. J.79(2), 962-974. Abstract
"Temperature dependence of force, velocity and processivity of single kinesin molecules."
Kawaguchi,K. and Ishiwata,S. (2000).
Biochem. Biophys. Res. Commun. 272(3), 895-899.Abstract
"Position-specific release of DNA from a chip by using photothermal denaturation."
Okano,K., Yasuda,K. and Ishiwata,S. (2000).
Sensors & Actuators B 64, 88-94. Abstract
"Temperature change does not affect force between single actin filaments and HMM from rabbit muscles."
Kawai,M., Kawaguchi,K., Saito,M. and Ishiwata,S. (2000).
Biophys. J. 78, 3112-3119.Abstract
"Focal extraction of surface-bound DNA from a microchip using photo-thermal denaturation."
Yasuda,K., Okano,K. and Ishiwata,S. (2000).
Biotechniques 28, 1006-1011. Abstract
"Effects of MgADP on length dependence of tension generation in skinned rat cardiac muscle."
Fukuda,N., Kajiwara,H., Ishiwata,S. and Kurihara,S. (2000).
Circ. Res. 86, e1-e6. Abstract
1999
"Tropomyosin modulates pH dependence of isometric tension."
Fujita,H. and Ishiwata,S. (1999).
Biophys. J. 77, 1540-1546. Abstract
"Imaging of thermal activation of actomyosin motors."
Kato,H., Nishizaka,T., Iga,T., Kinosita,Jr.,K. and Ishiwata,S. (1999).
Proc. Natl. Acad. Sci. USA 96, 9602-9606.Abstract PDF(367KB)
"Effects of pH on spontaneous tension oscillation in skinned bovine cardiac muscle."
Fukuda,N. and Ishiwata,S. (1999).
Pflugers Arch. 438, 125-132. Abstract
1998
"Regulatory roles of MgADP and calcium in tension development of skinned cardiac muscle."
Fukuda,N., Fujita,H., Fujita,T. and Ishiwata,S.(1998).
J.Muscle Res.Cell Motil. 19, 909-921. Abstract
"Contractile properties of thin (actin) filament-reconstituted muscle fibers."
Ishiwata,S., Funatsu,T. and Fujita,H. (1998).
Adv.Exp.Med.Biol. 453, 319-329.
"Spontaneous oscillatory contraction without regulatory proteins in actin filament-reconstituted fibers."
Fujita,H. and Ishiwata,S. (1998).
Biophys.J. 75, 1439-1445. Abstract
"F1-ATPase: A rotary motor made of a single molecule."
Kinosita,Jr.,K., Yasuda,R., Noji,H., Ishiwata,S. and Yoshida,M. (1998).
Cell 93,21-24.Abstract
"Use of fluorescent probes."
Ishiwata,S. (1998).
In Current Methods in Muscle Physiology-Advantages, Problems and Limitations-, Oxford Univ. Press. pp.199-222.
1997
"Inter-head distances in myosin attached to F-actin estimated by fluorescence energy transfer spectroscopy."
Ishiwata,S., Miki,M., Shin,I., Funatsu,T., Yasuda,K. and dos Remedios,C.G. (1997).
Biophys.J. 73, 895-904.Abstract
"Axial rotation of sliding actin filaments revealed by single-fluorophore imaging."
Sase,I., Miyata,H., Ishiwata,S. and Kinosita,Jr.,K. (1997). Proc.Natl.Acad.Sci.U.S.A. 94, 5646-5650. Abstract
"Two-dimensional arrangement of a functional protein by cysteine-gold substrate interaction: Enzyme activity and characterization of a protein monolayer on a gold substrate."
Sasaki,Y.C.,Yasuda,K.,Suzuki,Y.,Ishibashi,T.,Satoh,I.,Fujiki,Y. and Ishiwata,S.(1997).
Biophys.J. 72. 1842-1848. Abstract
1996
"Microscopic analysis of the nature of forces in a single actomyosin motor and its assemblage."
Ishiwata,S., Nishizaka,T., Kato,H., Tadakuma,H., Iga,T., Suzuki,N., Miyata,H. and Kinosita,Jr.,K. (1996).
Neurochem.(Moscow) 13, 305-313.
"Spontaneous tension oscillation in skinned bovine cardiac muscle."
Fukuda,N., Fujita,H., Fujita,T. and Ishiwata,S. (1996).
Pflug.Arch. 433, 1-8.Abstract
"Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle."
Fujita,H., Yasuda,K., Niitsu,S., Funatsu,T. and Ishiwata,S. (1996).
Biophys.J. 71,2307-2318.Abstract
"Ca2+ -induced tension development in the stalks of glycerinated Vorticella
convallaria."
Moriyama,Y., Yasuda,K., Ishiwata,S. and Asai,H. (1996).
Cell Motil.Cytoskel. 34,271-278. Abstract
"Soft-X-ray damage to biological samples."
Fujisaki,H., Takahashi,S., Ohzeki,H., Sugisaki,K., Kondo,H., Nagata,H., Kato,H. and Ishiwata,S.(1996).
J.Microscopy 182,79-84. Abstract
"Microscopic analysis of the elastic properties of connectin/titin and nebulin in myofibrils."
Ishiwata,S., Yasuda,K., Shindo,Y. and Fujita,H. (1996).
Adv.Biophys. 33, 135-142.Abstract
"Synchronous behavior of spontaneous oscillations of sarcomeres in skeletal
myofibrils under isotonic conditions."
Yasuda,K., Shindo,Y. and Ishiwata,S. (1996).
Biophys.J. 70, 1823-1829.Abstract
"Preparation of bead-tailed actin filaments: Estimation of the torque produced by the sliding force in an in vitro motility assay."
Suzuki,N., Miyata,H., Ishiwata,S. and Kinosita,Jr.,K. (1996).
Biophys.J. 70, 401-408.Abstract
1995
"Unbinding force of a single motor molecule of muscle measured using optical tweezers."
Nishizaka,T., Miyata,H., Yoshikawa,H., Ishiwata,S. and Kinosita,Jr.,K.(1995).
Nature 377, 251-254. Abstract Go to movie page.
"Mechanical measurements of single actomyosin motor force."
Miyata,T., Hakozaki,H., Yoshikawa,H., Suzuki,N., Furuno,T., Ikegami,A., Kinosita,Jr.,K., Nishizaka,T. and Ishiwata,S.(1995).
Biophys.J. 68, 286s-290s.Abstract
"Mechanical properties of a single protein motor of muscle studied by optical tweezers."
Nishizaka,T.,Miyata,T.,Yoshikawa,H.,Ishiwata.S. and Kinosita,Jr.,K. (1995).
Biophys.J. 68, 75s. Abstract
"Microscopic analysis of the elastic properties of nebulin in skeletal myofibrils."
Yasuda,K., Anazawa,T. and Ishiwata,S. (1995).
Biophys.J. 68, 598-608.Abstract
1994
"Length regulation of thin filaments without nebulin."
Yasuda,K., Fujita,H., Fujiki,Y. and Ishiwata,S. (1994).
Proc.Jap.Acad. 70, Ser.B. .151-156.
"Microscopic measurement of the sliding and binding force between muscle proteins with optical tweezers."
Nishizaka,T., Miyata,H., Yoshikawa,H., Ishiwata,S. and Kinosita,Jr.,K. (1994).
in Optical methods in biomedical and environmental sciences. (ed. by Ohzu,H. & Komatsu,S.) Elsevier Sci.,B.V. pp.195-198.
"Stepwise motion of an actin filament over a small number of heavy meromyosin
molecules is revealed in an in vitro motility assay."
Miyata,H., Hakozaki,H., Yoshikawa,H., Suzuki,N., Kinosita,Jr.,K., Nishizaka,T. and Ishiwata,S. (1994).
J.Biochem. 115, 644-647.Abstract
"Structural and functional reconstitution of thin filaments in skeletal muscle.
"Funatsu,T., Anazawa,T. and Ishiwata,S. (1994).
J. Muscle Res.Cell Motil. 15, 158-171. Abstract
1993
"Mechano-chemical coupling in spontaneous oscillatory contraction of muscle."
Ishiwata,S. and Yasuda,K. (1993).
Phase Transitions 45, 105-136.
"Spontaneous tension oscillation (SPOC) of muscle fibers and myofibrils.
Minimum requirements for SPOC."
Ishiwata,S.,Anazawa,T.,Fujita,T.,Fukuda,N.,Shimizu,H. and Yasuda,K. (1993). In Mechanism of myofilament sliding in muscle contraction. (ed. by H.Sugi & G.H.Pollack) pp.545-556. Abstract
"Orientation of actin monomers in moving actin filaments."
Kinosita,Jr.,K., Suzuki,N., Ishiwata,S., Nishizaka,T., Itoh,H., Hakozaki,H., Marriott,G. and Miyata,H. (1993).
In Mechanism of myofilament sliding in muscle contraction. (ed. by H.Sugi & G.H.Pollack) pp.321-329.
"Changes in mobility of chromaffin granules in actin network with its assembly and Ca2+ -dependent disassembly by gelsolin."Miyamoto,S., Funatsu,T., Ishiwata,S. and Fujime,S. (1993). Biophys.J. 64, 1139-1149.Abstract
"Elastic filaments in situ in cardiac muscle: Deep-etch replica analysis in combination with selective removal of actin and myosin filaments."
Funatsu,T., Kono,E., Higuchi,H., Kimura,S., Ishiwata,S., Yoshioka,T., Maruyama,K. and Tsukita,S. (1993).
J.Cell Biol. 120, 711-724.Abstract
"Right-handed rotation of an actin filament in an in vitro motile system."
Nishizaka,T.,Yagi,T.,Tanaka,Y. & Ishiwata,S. (1993)
Nature 361, 269-271.
1992
"A dynamic light scattering study on changes in mobility of chromaffin granules inactin network with its assembly and Ca?2 + -dependent disassembly by gelsolin."
Fujime,S., Miyamoto,S., Funatsu,T. and Ishiwata,S. (1992).
SPIE 1922, 337-346.Abstract
"Super helix formation of actin filaments in an in vitro motile system."
Tanaka,Y.,Ishijima,A. and Ishiwata,S. (1992)
Biochim.Biophys.Acta 1159, 94-98.Abstract
"Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils.
Microscopic measurement and analysis."
Anazawa,T., Yasuda,K. and Ishiwata,S. (1992)
Biophys.J. 61, 1099-1108.Abstract
"Regulation of tension development by MgADP and Pi without Ca2+ Role in
spontaneous tension oscillation of skeletal muscle."
Shimizu,H., Fujita,T. and Ishiwata,S. (1992)
Biophys.J. 61, 1087-1096.Abstract
1991
"Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium."
Kinosita,Jr.,K., Itoh,H., Ishiwata,S., Hirano,K., Nishizaka,T. and Hayakawa,T. (1991)
J.Cell Biol. 115,67-73. Abstract
"Spontaneous oscillatory contraction (SPOC) of sarcomeres in skeletal muscle."
Ishiwata,S., Okamura,N., Shimizu,H., Anazawa,T. and Yasuda,K. (1991)
Adv.Biophys. 27, 227-235.Abstract
"Scanning tunneling microscopy of actin filament."
Masai,J., Shibata,T., Kondo,S. and Ishiwata,S. (1991)
J.Vac.Sci.Tech. B 9, 1177-1179.Abstract
1986~1990
"Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin."
Funatsu,T., Higuchi,H. and Ishiwata,S. (1990)
J. Cell Biol. 110, 53-62.Abstract
"Diffraction rings obtained from a suspension of skeletal myofibrils by laser light illumination. Study of internal structure of sarcomeres."
Ishiwata,S. and Okamura,N. (1989)
Biophys.J. 56, 1113-1120. Abstract
"Optical anisotropy decay studies of the dynamic structure of myosin filaments."
Ishiwata,S.,Kinosita,Jr.,K.,Yoshimura,H. and Ikegami,A. (1988)
In Molecular mechanism of muscle contraction. (ed. by H.Sugi & G.H. Pollack) (Plenum Pub.Corp.). pp.267-276.Abstract
"Rotational motions of myosin head in solution and in myofibrils studied by time-
resolved optical anisotropy decay measurements."
Ishiwata,S., Kinosita,Jr.,K., Yoshimura,H., Asai,H. and Ikegami,A. (1988)
In Energy transduction in ATPases. (ed. by Y.Mukohata,M.F.Morales & S.Fleischer)(Yamada Science Foundation). pp.26-29.
"Spontaneous oscillatory contraction of sarcomeres in skeletal myofibrils."
Okamura,N. and Ishiwata,S. (1988)
J.Muscle Res.Cell Motil. 9, 111-119.Abstract
"s-Actinin isoforms in various types of muscle and non-muscle tissues."
Asami,Y., Funatsu,T. and Ishiwata,S. (1988)
J.Biochem. 103, 76-80.
"Transition of s-actinin isoforms during development of chicken skeletal muscle."
Asami,Y., Funatsu,T. and Ishiwata,S. (1988)
J.Biochem. 103, 72-75.
"s-Actinin: A capping protein at the pointed end of thin filaments in skeletal muscle."
Funatsu,T., Asami,Y. and Ishiwata,S. (1988)
J.Biochem. 103, 61-71. Abstract
"Electro-optic effect of single muscle fibers."
Fujime,S., Takasaki-Ohsita,M. and Ishiwata,S. (1987)
Rep.Prog.Polym.Phys.Japan XXX, 799-802.
"Dynamic light-scattering study of muscle F-actin II."
Fujime,S., Takasaki-Ohsita,M. and Ishiwata,S. (1987)
Biophys.Chem. 27, 211-224
"s-Actinin is not distinguishable from an actin barbed-end capping protein in chicken breast muscle."
Oosawa,M., Shimaoka,S., Funatsu,T., Ishiwata,S. and Maruyama,K. (1987)
J.Biochem. 101, 1481-1483.
"Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements."
Ishiwata,S., Kinosita,Jr.K., Yoshimura,H. and Ikegami,A. (1987)
J.Biol.Chem. 262, 8314-8317. Abstract
"Dynamic light-scattering study on polymerization process of muscle actin."
Masai,J., Ishiwata,S. and Fujime,S. (1986)
Biophys.Chem. 25, 253-269
"Accumulated strain mechanism for length determination of thick filaments in skeletal muscle. I. Experimental bases."
Higuchi,H., Funatsu,T., Ishijima,A., Okamura,N. and Ishiwata,S. (1986)
J.Muscle Res. Cell Motil. 7, 491-500.
"Saturation transfer electron paramagnetic resonance study of the mobility of myosin heads in myofibrils under conditions of partial dissociation."
Ishiwata,S., Manuck,B.A., Seidel,J.C. and Gergely,J. (1986)
Biophys.J. 49, 821-828.
1981~1985
"Assembly and disassembly of actin filaments in myofibrils."
Ishiwata,S., Funatsu,T. and Asami,Y.(1985)
ibid. (Taniguchi Foundation).pp.122-131.
"Optical anisotropy decay studies of the dynamics of muscle proteins."
Kinosita,Jr.K., Ishiwata,S., Yoshimura,H. and Ikegami,A.(1985)
Proc.11th Taniguchi Int. Symp. In Actin: Structure and functions. (ed. by T. Yanagida)(Taniguchi Foundation). pp.32-45.
"Characterization of s-actinin: A suppressor of the elongation at the pointed end of thin filaments in skeletal muscle."
Funatsu,T. and Ishiwata,S. (1985)
J.Biochem. 98, 535-544. Abstract
"Disassembly kinetics of thick filaments in rabbit skeletal muscle fibers. Effects of ionic strength, Ca2+ concentration, pH, temperature, and cross-bridges on the stability of thick filament structure."
Higuchi,H. and Ishiwata,S. (1985)
Biophys.J. 47, 267-275.
"Disassembly from both ends of thick filaments in rabbit skeletal muscle fibers.
An optical diffraction study."
Ishiwata,S.,Muramatsu,K. and Higuchi,H. (1985)
Biophys.J. 47,257-266.
"Does actin bind to the ends of thin filaments in skeletal muscle?"
Ishiwata,S. and Funatsu,T. (1985)
J.Cell Biol. 100, 282-291.
"Submicrosecond and microsecond rotational motions of myosin head in
solution and in myosin synthetic filaments as revealed by time-resolved optical
anisotropy decay measurements."
Kinosita,Jr.K.,Ishiwata,S.,Yoshimura,H.,Asai,H. and Ikegami,A. (1984)
Biochemistry 23, 5963-5975.
"Dynamic light scattering study of muscle F-actin."
Fujime,S., Ishiwata,S. and Maeda,T. (1984)
Biophys.Chem. 20, 1-21.
"Dynamic light scattering study of muscle F-actin in solution."
Fujime,S., Ishiwata,S. and Maeda,T.(1983)
In The application of laser light scattering to the study of biological motion. (ed. by J.C. Earnshaw & M.W.Steer)(Plenum Pub.Corp.). pp.459-475.
"F-actin and thin filament of muscle studied by laser light scattering."
Fujime,S., Maeda,T. and Ishiwata,S. (1982)
In Biomedical applications of laser light scattering. (ed. by D.B.Sattelle,W.I.Lee & B.R.Ware)(Elsevier Biomedical Press). pp.251-269.
"Melting from both ends of A-band in a myofibril. Observation with a phase-contrast microscope."
Ishiwata,S. (1981)
J.Biochem. 89, 1647-1650. Abstract
~1980
"Partial destruction and reconstruction of an ordered structure in striated muscle."Ishiwata,S. (1980)
Proc.6th Taniguchi Int.Symp. In Protein dynamics and energy transduction. (ed. by S.Ishiwata)(Taniguchi Foundation). pp.262-293.
"Submillisecond rotational dynamics of spin-labeled myosin heads in myofibrils."
Thomas,D.D., Ishiwata,S., Seidel,J.C. and Gergely,J. (1980)
Biophys.J. 48, 873-890.
"Studies on the F-actin-tropomyosin-troponin complex. III. Effects of troponin components and calcium ion on the binding affinity between tropomyosin and F-actin."
Ishiwata,S. (1978)
Biochim.Biophys.Acta 534, 350-357.
"Studies on the F-actin-tropomyosin-troponin complex. II. Partial reconstitution of thin filament by F-actin, tropomyosin and tropomyosin binding component of troponin (TNT)."
Ishiwata,S. and Kondo,H. (1978)
Biochim.Biophys.Acta 534, 341-349.
"Dynamic characteristics of F-actin and thin filaments in vivo and in vitro."
Oosawa,F., Maeda,Y., Fujime,S., Ishiwata,S., Yanagida,T. and Taniguchi,M. (1977)
J.Mechanochem.Cell Motility 4, 63-78.
"Critical behavior of density fluctuations in gels."
Tanaka,T., Ishiwata,S. and Ishimoto,C. (1977)
Phys.Rev.Lett. 38, 771-774. Abstract
"Freezing of actin. Reversible oxidation of a sulfhydryl group and structural change."
Ishiwata,S. (1976)
J.Biochem. 80, 595-609. Abstract
"Uni-directional growth of F-actin."
Kondo,H. and Ishiwata,S. (1976)
J.Biochem. 79, 159-171. Abstract
"A regulatory mechanism of muscle contraction based on the flexibility change of the thin filament"
Ishiwata,S. and Oosawa,F. (1974)
J.Mechanochem.Cell Motility 3, 9-17.
"Light-beating study of the effect of s-actinin on the interaction between F-actin and heavy-meromyosin."
Maeda,T., Ishiwata,S. and Fujime,S. (1974)
Biochim.Biophys.Acta 336, 445-452.
"Effect of calcium ion on the thin filament in muscle."
Oosawa,F., Yanagida,T., Maeda,T., Ishiwata,S., Fujime,S. and Mihashi,K. (1974)
In Calcium binding protein (ed. by Drabikowski et al.) (BBA Library).pp.203-221.
"A study on the F-actin, tropomyosin and troponin complex. I. Gel-filament transformation."
Ishiwata,S. (1973)
Biochim.Biophys.Acta 303, 77-89.
"Dynamic property of F-actin and thin filament."
Oosawa,F., Fujime,S., Ishiwata,S. and Mihashi,K. (1972)
Cold Spr.Harb.Symp.Quant. Biol. 37, 277-285.
"F-actin-heavy meromyosin complex studied by optical homodyne and heterodyne methods"Fujime,S., Ishiwata,S. and Maeda,T. (1972)
Biochim.Biophys.Acta 283, 351-363.Abstract
"Effect of calcium ions on the flexibility of reconstituted thin filaments of muscle studied by quasielastic scattering of laser light."
Ishiwata,S. and Fujime,S. (1972)
J.Mol.Biol. 68, 511-522. Abstract
"Effect of Ca2+ on dynamic properties of muscle proteins studied by quasielastic light scattering."
Ishiwata,S. and Fujime,S.(1971c)
J.Phys.Soc.Japan 31,1601.
"Dynamic study of F-actin by quasielastic scattering of laser light."
Fujime,S. and Ishiwata,S. (1971)
J.Mol.Biol. 62, 251-265.Abstract
"Effect of Ca2+ on the F-actin-tropomyosin-troponin complex studied by quasielastic light scattering."
Ishiwata,S. and Fujime,S. (1971b)
J.Phys.Soc.Japan 30, 303.
"A dynamic study of F-actin-tropomyosin solutions by quasielastic light scattering."
Ishiwata,S. and Fujime,S. (1971a)
J.Phys.Soc.Japan 30, 302.
"A dynamic study of F-actin-heavy meromyosin solutions by quasielastic light scattering."
Fujime,S. and Ishiwata,S.(1970)
J.Phys.Soc.Japan 29,1651.
"Study on muscle proteins -Principally, dynamic properties of actin filament studied by quasielastic scattering of laser light."
Ishiwata,S. (1975)
D.Sc.Thesis, Nagoya Univ.